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SDS-PAGE characterization of cross-linked A-I/A-II dHDL. SDS-PAGE (8–25%, Phast gel, GE Healthcare) of A-I/A-II dHDL (lane 1), and A-I/A-II dHDL upon cross-linking with 1:100 total protein:BS3 (lane 2). GE Healthcare low molecular weight standards are shown in lane 3. Band a, ∼28 kDa is apoA-I; band b, ∼60 kDa is two apoA-I molecules cross-linked together; band c, ∼79 kDa is a trimer consisting of two apoA-I and one apoA-II. The gel was stained with Coomassie Blue.
