FIGURE 8.

Proteolytic patterns of A-I dHDL and A-I/A-II dHDL. Particles were subjected to limited proteolytic digestions under similar conditions as reported before (11). Digestions were carried out at 1:200 total protein to trypsin ratio at 37 °C for 2 h. Digestion was quenched by the addition of SDS sample loading buffer followed by boiling for 3 min. Reaction mixtures were analyzed on a 8–25% SDS-Phast gel. Lane 2, A-I dHDL; lane 3, A-I dHDL subjected to proteolysis; lane 4, A-I/A-II dHDL; lane 5, A-I/A-II dHDL subjected to proteolysis. Molecular mass markers (Bio-Rad) are on lane 1.