FIGURE 11.

Macrophage parasitemia and mouse infectivity. A, mouse peritoneal macrophages were infected with wild type, Δaah, Δaah[pAAH], Δhgprt/Δxprt, Δaah/Δhgprt/Δxprt, or Δaah/Δhgprt/Δxprt[pXPRT] stationary phase promastigotes at a multiplicity of infection of 10 parasites/macrophage. Cells were stained after 72 h and amastigotes enumerated visually. The results are the averages and standard errors of three independent determinations (n = 3) plotted on a log scale. B, three separate groups of five BALB/c mice were infected with wild type (1), Δaah (2), or Δaah/Δhgprt/Δxprt (3) late log phase promastigotes. Mice were sacrificed 4 weeks post-infection, and parasite loads in livers and spleens quantified using limiting dilution.