FIGURE 4.

Immunoblotting of cellular fractionations. A, L. donovani promastigote cell lysates were fractionated by sedimentation at 45,000 × g as described previously under “Experimental Procedures,” and the cytosolic (C) (lane 1) and organellar (O) (lane 2) fractions were subjected to Western blot analysis using anti-AAH, anti-APRT, or anti-IMPDH antisera. B, the organellar pellet obtained by sedimenting the L. donovani cell lysate at 45,000 × g was subjected to sucrose density centrifugation, and fractions were collected. Each fraction from the sucrose gradient was then subjected to Western blot analysis using anti-AAH and anti-IMPDH antibodies. The cytosolic and gradient portions are indicated at the top of the panel.