FIGURE 7.

Expression of AAH in Leishmania life cycle stages. Lysates prepared from wild type (lanes 1 and 2), Δaah (lanes 3 and 4), and Δaah[pAAH] (lanes 5 and 6), promastigotes (P) (lanes 1, 3, and 5), and axenic amastigotes (A) (lanes 2, 4, and 6) were analyzed by Western blotting using mouse monoclonal antibodies raised against the amastigote-specific A2 protein family and polyclonal rabbit anti-AAH antibodies. The blots were also probed with polyclonal rabbit anti-HGPRT antibodies as loading controls.