FIGURE 8.

AAH activity in intact parasites. Intact wild type (lanes 1 and 2) and Δaah (lanes 3 and 4) promastigotes (P) and axenic amastigotes (A) were incubated with 67 μm [¹⁴C]adenine, and the production of [¹⁴C]hypoxanthine was monitored by TLC over a 1-h time course. The ability of wild type promastigotes and axenic amastigotes to convert [¹⁴C]adenine into [¹⁴C]hypoxanthine in the presence of 0.67 mm dCF was also assessed (lanes 5 and 6). The 30-min time point is depicted for each sample. Standards are shown to the left of the chromatograms as follows: Ade, adenine; Hyp, hypoxanthine; and AMP.