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Purification of recombinant L. donovani AAH protein and assessment of substrate specificity. The ability of a catalytic excess of purified AAH to deaminate [14C]adenine or [14C]adenosine was evaluated by the TLC methodology described under “Experimental Procedures.” The 0- and 2-h time points for each sample are shown. Standards are shown on the left of each chromatogram: Ade, adenine; Hyp, hypoxanthine; Ado, adenosine; and Ino, inosine.
