FIGURE 2.

EGFR activation alters K17 solubility. A, growth factor-deprived A431 cells were treated with (+EGF) or without (-EGF) 100 ng/ml EGF for 1 h and then immunostained with an anti-K17 antibody. Scale bar = 20 μm. Boxed-in areas are enlarged on the right. B, growth factor-deprived A431 cells were treated with 100 ng/ml EGF for the indicated time periods. Whole cell lysates were processed for Triton solubility. Immunoblotting was performed with antibodies against the indicated proteins. C, K17 and K5 signal intensities from B were quantified using the AlphaView SA software. TS indicates Triton soluble, whereas IS indicates Triton insoluble pool. *, p < 0.006; **, p < 0.0003 compared with 0 min TS.