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. 2012 Jan 10;287(10):7573–7581. doi: 10.1074/jbc.M111.301549

FIGURE 5.

FIGURE 5.

AnxA2 stabilizes keratin filaments and delays EGF-induced change in keratin solubility. A–D, mCherry-AnxA2 (AnxA2) or vector control (V) was transiently transfected into A431 cells. A, whole cell lysates were processed for Triton solubility. B, quantitation of A. Relative K17 levels in TS/IS for cells expressing vector control or mCherry-AnxA2 are shown. *, p < 0.05. C, growth factor-deprived cells were treated with 100 ng/ml EGF for the indicated time periods, and whole cell lysates were prepared in Triton lysis buffer. Immunoblotting was performed with antibodies against the indicated proteins, and signal intensities were quantified using AlphaView SA. D, quantitation of C. Relative K17 levels were normalized to those from 0 min EGF stimulation. *, p <0.05. E, whole cell lysates of A431 cells stably expressing vector control or AnxA2 shRNA were processed for Triton solubility. F, quantitation of E. Relative K17 levels in TS/IS are shown. *, p <0.05.