Figure 5.

Correction of the GPIbα^null bleeding phenotype by 2bIbα lentiviral vector (LV)-transduced bone marrow transplantation (BMT). 2bIbα LV-transduced and untransduced hematopoietic stem cells (HSC) recipients were analyzed by tail bleeding time assays 10 weeks after transplantation. 2bIbα (1°) represents primary recipients that were transplanted with 2bIbα LV-transduced BM (n = 9), and 2bIbα (2°) represents secondary recipients that that received BMT from primary recipients. When bleeding did not cease within 10 minutes, the tail was cauterized and bleeding time was recorded as 600 seconds. Wild-type C57BL/6J and GPIbα^null mice were used as controls. Prolonged bleeding time of GPIbα^null is rescued in both 2bIbα LV-transduced (1°) and 2bIbα LV-transduced (2°) recipients.