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. 2011 Dec 20;20(3):572–579. doi: 10.1038/mt.2011.280

Figure 1.

Figure 1

AAV vector-induced gag-specific CD8+ T cell responses. Groups of 5–10 BALB/c mice were injected intramuscularly with the indicated doses of AAV or Ad vectors expressing gag. (a) Frequencies of gag-specific CD8+ T cells were assessed from blood at different times after injection of vector by staining with an antibody to CD8 and the gag-tetramer. Graphs show average frequencies of tetramer+ CD8+ cells over all CD8+ cells ± SE. Student's t-tests were used to determine differences between T cell responses to ss and scAAV vectors at each individual time point. *,** indicate significant differences (*P < 0.05, **P < 0.01). (b) Frequencies of gag-specific CD8+ T cells measured as described in a before (d0) and at different times (week 2 and 4) after a booster immunization with an Ad vector expressing gag. Differences between responses in AAV-primed versus unprimed mice following Ad boost were not significant (P > 0.05) but for the comparison between ssAAV7 prime + Ad boost and Ad boost only at week 4 (P < 0.01). AAV, adeno-associated virus; Ad, adenovirus; scAAV, self-complementary AAV; ssAAV; single-stranded AAV; vg, vector genome.