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. 2011 Dec 20;20(3):572–579. doi: 10.1038/mt.2011.280

Figure 2.

Figure 2

Cytokine/chemokine production by AAV vector-induced gag-specific CD8+ T cells. Groups of 10 BALB/c mice were immunized with the indicated vectors. Three weeks later PBMCs were tested by ICS for cytokine or chemokine production in response to the gag peptide AMQMLKETI. Cells in control wells were stimulated with an unrelated peptide. Frequencies of cytokine-producing CD8+ T cells were low and plots were therefore concatenated to allow for more accurate gating. (a) Shows frequencies of CD8+ T cells with individual mice producing any of the four cytokines/chemokines and combinations thereof tested. Filled squares indicate responses to gag, unfilled squares for control wells. (b) Shows the percentages of cytokine/chemokine positive cells producing either of the four cytokines/chemokines. AAV, adeno-associated virus; ICS, intracellular cytokine staining; IFN, interferon; IL, interleukin; MIP, macrophage inflammatory protein; PBMC, peripheral blood mononuclear cell; TNF, tumor necrosis factor; vg, vector genome.