Figure 2.

Primary Crh transcripts show graded responses in the PVH after glycemic challenges. a, Photomicrographs of CRH hnRNA hybridization signals show that levels increase in the PVHmpd after intravenous injections of insulin (Ins) or 2-DG but not saline (Sal). bi–biii, No AVP hnRNA hybridization was seen in the PVHmpd after any treatment). biv, In situ hybridization signal for AVP hnRNA in an adrenalectomized (Adx) rat (for comparison). Note the presence of robust signal in both PVH compartments of the adrenalectomized rat, whereas those in bi–biii lack detectable AVP hnRNA signal in the PVHmpd. c, CRH hnRNA responses were significantly greater for 2-DG than for insulin. ***p < 0.0001. Numbers below each bar are the samples per group.