Figure 5.

Increased Crh transcription following glycemic challenges is dependent on intact catecholaminergic inputs to the neuroendocrine PVH. a, Insulin or 2-DG significantly increased mean (+SEM) CRH hnRNA levels compared with saline (Sal) in the PVHmpd of sham (MSAP)-lesioned rats (black bars). Catecholaminergic denervation of the PVH completely abolished the Crh transcriptional response to insulin (white bars) and significantly attenuated the response after 2-DG, whereas partial lesions (gray bars) had no effect on Crh transcriptional responses. **p < 0.001, ***p < 0.0001. ns, Not significant. Numbers below the bars are the samples per group. b, Representative photomicrographs of the CRH hnRNA in situ hybridization signals in animals with sham or complete lesions following insulin or 2-DG injections.