Table 2. Data collection and refinement statistics.
Native | Lutetium | Dysprosium | |
---|---|---|---|
Data collection | |||
Space group | P21212 | P21212 | P21212 |
Cell dimensions | |||
a, b, c (Å) | 102.5, 208.7, 36.9 | 104.9, 207.6, 36.8 | 102.6, 208.2, 37.0 |
α, β, γ (°) | 90.0, 90.0, 90.0 | 90.0, 90.0, 90.0 | 90.0, 90.0, 90.0 |
Wavelength (Å) | 0.93930 | 1.13743 | 1.36242 |
Resolution (Å) | 19.7–2.25 (2.36–2.25)* | 40.0–3.21 (3.51–3.21) | 40.0–3.6 (3.79–3.60) |
Rmerge | 5.7 (53.3) | 13.7 (58.2) | 19.7 (51.9) |
I/σI | 10.7 (2.1) | 7.0 (2.1) | 10.5 (4.9) |
Completeness (%) | 97.2 (97.2) | 91.5 (72.4) [80.6]† | 99.9 (99.8) [99.8] |
Redundancy | 2.5 (2.5) | 4.9 (4.7) [2.8] | 12.6 (10.8) [5.9] |
Refinement | |||
Resolution (Å) | 2.25 (2.31–2.25) | ||
No. of reflections | 14,648 (997) | ||
Rwork/Rfree | 22.3/26.3 (25.2/34.2) | ||
No. of atoms§ | 4,120 | ||
Protein | 4,019 | ||
Water | 93 | ||
Calcium | 5 | ||
Sodium | 3 | ||
Mean B-factor (Å2) | 11.7 | ||
R.m.s. deviations | |||
Bond lengths (Å) | 0.010 | ||
Bond angles (°) |
1.224 |
|
|
*Values in parentheses are for highest-resolution shell.
†Values in square brackets are for anomalous statistics.
§Two EG complexes/asymmetric unit.