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. 2011 Nov 29;20(3):633–643. doi: 10.1038/mt.2011.256

Figure 1.

Figure 1

Generation and specific immune recognition by P4 anti-mesothelin CAR-transduced human T cells in vitro. (a) Schematic representation of P4 based chimeric antigen receptor (CAR) constructs containing the CD3ζ cytosolic domain alone (P4-z) or in combination with the CD28 costimulatory module (P4-28z). The anti-CD19-z and anti-CD19-28z CARs are shown. (b) P4 CAR expression (filled black histograms) was detected on human CD3-gated cells via recombinant mesothelin protein staining after transduction with lentivirus compared to untransduced T cells (open histograms). Anti-CD19 transduced T cells were detected using protein L followed by SA-PE. Transduction efficiencies are indicated with the percentage of CAR expression and the mean fluorescence intensity of the transduced populations in parentheses. L, linker; P4, antimesothelin scFv; VH, variable H chain; VL, variable L chain; SA-PE, streptavidin-phycoerythrin; scFv, single-chain antibody variable fragment; TM, transmembrane region.