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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Biochim Biophys Acta. 2011 Dec 10;1821(3):425–434. doi: 10.1016/j.bbalip.2011.11.012

Fig. 5.

Fig. 5

Macrophage analysis of abdominal adipose tissue and resident peritoneal leukocytes taken from WT, AL and CR mice at 12 weeks of age. (A) Abdominal adipose tissue was collected from mice, digested, and centrifuged to provide a buoyant adipocyte-enriched cell population and a pellet of stromal vascular cells (SVCs). The SVCs were labeled with antibodies to CD11c and F4/80 and isolated using FACS. Quantitative RT-PCR was used to determine the relative levels of ABCG1 in each fraction. ABCG1 expression was most abundant in F4/80+ cell fractions. (B, C) Resident peritoneal macrophages were isolated (i.e. without thioglycollate treatment) from AL and CR db/db mice at 12 weeks and examined for (B) macrophage markers and (C) cholesterol metabolism genes. Total cell counts were not determined and thus, mRNA levels represent the relative expression of all collected cells. Significant differences in cell phenotypes are seen. (D) Total cholesterol content of resident peritoneal leukocytes taken from AL and CR mice at 12 weeks of age. †p<0.05 between diet groups, n=8.