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Immunostaining of EAATs in sub-confluent human astrocytoma cells. Sub-confluent STTG-1 cells were labelled for EAAT1/GLAST (A, B and E, F) or EAAT2/GLT-1 (C, D and G, H) using primary antibodies obtained from Santa Cruz (directed against N-terminal part of GLAST or GLT-1) (A and C) or from Chemicon (directed against the C- terminal part of GLAST or GLT-1) (E and G). Nuclei were stained with DAPI (B, D, F, H).
