Table 3.
Net transport of L-glutamate by human cancer cells and rat astrocytes.
| Sub-confluent cells | Confluent cells | |||||
|---|---|---|---|---|---|---|
| control | + PDC | + Sulfa | control | + PDC | + Sulfa | |
| AGS | + 32 (± 15) | + 39 (± 21) | - 9.1 (± 13) | - 74 (± 6.1) | + 41 (± 18) | - 81.2 (± 8.4) |
| Caco-2 | + 36 (± 26) | + 44 (± 18) | - 4.5 (± 9.2) | - 69.9 (± 9.6) | + 42 (± 12) | -72.6 (± 9.6) |
| HeLa | + 27 (± 12) | + 39 (± 9.8) | - 7.8 (± 11) | - 63.2 (± 5.8) | + 47 (± 18) | - 69.1 (± 5.2) |
| HOG | + 37 (± 9.6) | + 41 (± 12) | - 2.3 (± 3.4) | - 68.4 (± 9.4) | + 52 (± 17) | -71.7 (± 6.8) |
| HT29 | + 48 (± 14) | + 51 (± 7.5) | - 5.4 (± 9.1) | - 64.1 (± 14) | + 49 (± 9.8) | - 69.8 (± 5.9) |
| SHSY5Y | + 15 (± 9.9) | + 34 (± 6.4) | - 13 (± 6.8) | - 74 (± 1.8) | + 48 (± 13) | - 79.2 (± 9.4) |
| STTG-1 | + 52 (± 24) | + 62 (± 13) | - 6.9 (± 3.4) | + 69 (± 12) | + 74 (± 9.5) | - 9.1 (± 7.9) |
| Astrocytes | - 79 (± 2.1) | + 14 (± 10.9) | - 82 (± 3.4) | - 91.7 (± 2.7) | + 21 (± 12) | - 95.6 (± 9.1) |
Net transport of L-glutamate was measured as explained in Materials and Methods. Results were expressed as variations of the extracellular concentration of L-glutamate after 6 h of incubation at 37°C. Initial concentration of L-glutamate in cell medium was 100 μM. Positive and negative variation values corresponded to net secretion and absorption of L-glutamate, respectively. Inhibitors used were PDC at 100 μM and sulfasalazine at 250 μM. All results were obtained from three independent experiments and were expressed as means of the variation (in μM) ± standard deviation (S.D). Values in bold are statistically different from the control values with p at least < 0.05.