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. 2012 Mar 5;7(3):e32439. doi: 10.1371/journal.pone.0032439

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Abdgawad et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Neutrophils isolated from 60 HBD, 44 AASV patients, 8 PV patients, 18 TP, 21 SLE patients, and 20 RA patients were cultured in vitro in AIM-V medium. The percentage of surviving neutrophils (1a) and apoptotic neutrophils (1b) was measured after 20 hours. % Neutrophil survival = % of annexin-V negative and 7-AAD negative cells after 20 hour culture. % Neutrophil apoptosis = % of annexin-V positive and 7-AAD negative cells after 2o hour culture. HBD = healthy blood donors. AASV = ANCA-Associated Systemic Vasculitis. PV = Polycythemia Vera. TP = renal transplant recipients. SLE = Systemic Lupus Erythematosus. RA = Rheumatoid Arthritis.