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. 2012 Mar 5;7(3):e32431. doi: 10.1371/journal.pone.0032431

Figure 1. Mapping of influenza-specific CD8 T cell responses.

Figure 1

(A) Gating strategy and IFN-γ and TNF-α expression of CD8 T cells in response to influenza nucleoprotein (NP) from influenza vaccinated 19351, B0547 and B0527, were investigated first by stimulating whole blood with a pool of 98 17-mer peptides overlapping by 12 amino acids (NP peptide pool), then to pools of 10 17-mers and finally to individual 17-mers. A common response within the first 10 17-mers (NP peptides 1–10) was mapped to the third nucleoprotein peptide (NP peptide #3). (B) Peptide titration performed on the influenza-vaccinated animal B0547 at day 77 following the initial influenza vaccination. Whole blood was stimulated with 29 peptides (7 11-mers, 9 10-mers, 11 9-mers and 2 8-mers) at diminishing dilutions and responses measured by ICS assay. Peptides yielding responses above background are shown together with a representative negative peptide (ER9).