Figure 3. Positions of forward and reverse degenerate primers for the six R. microplus biogenic amine receptors.

The four amino acids comprising the degenerate tail of the primers are highlighted illustrating the gene specific nature of the primer design. Reverse primers are italicised and underlined. Examples of motifs highly conserved across receptor types, used for isolation by the alternative method of degenerate PCR and cloning, are in red. Sequences isolated in this manuscript are in bold.