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. 2012 Mar 5;7(3):e32551. doi: 10.1371/journal.pone.0032551

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Fu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Concentrations of individual unconjugated BAs in liver during aging and (B) proportions of them in all unconjugated BAs in liver at 3 and 27 months of age in male and female mice. Data are presented as means ± SEM of 5–7 mice. Daggers (†) represent statistically significant difference from the value at 3 months of age during aging of male mice. Double daggers (‡) represent significant difference from the value at 3 months of age during aging of female mice. Age-dependent differences were considered at p<0.05 by one-way ANOVA, followed by Duncan's post-hoc test. Asterisks (*) represent statistically significant difference between male and female mice at respective ages during aging (p<0.05), by student t-test. In panel B, pound signs (#) represent differences of BA proportions between 3 and 27 months.