Figure 2. Parasitemia (%) profile of Y-control line, selected resistant lines and release lines (inoculum 1×10⁸ parasites).

Drug resistance selection led to a loss of fitness and virulence as all the selected lines became not-lethal (A). Release of drug pressure partially restores fitness and virulence; lines could become lethal again (B). Parasitemia kinetic of the strains Y-ART3 (large square), Y-CQ2 (small square), Y-control (circle) and death of mice (cross) are shown. Figure 2 is one representative experiment from more than 5 experiments done with each of the strains tested.