Figure 2. Salt dependence of synRNB.

A. 5 nM of recombinant protein were incubated with 10 nM of Poly(A) at 37°C for 5 minutes in a reaction buffer with different salt concentrations (lanes 1 = 10 mM, lanes 2 = 50 mM, lanes 3 = 100 mM, lanes 4 = 150 mM, and lanes 5 = 200 mM). B. Determination of the protein activity in the presence of NaCl and KCl in different concentrations.