Figure 4. Divalent metal ion dependence of synRNB.

A. 10 nM of recombinant protein were incubated with 10 nM of Poly(A) at 37°C for 5 and 60 minutes in a reaction buffer with different divalent metal ions. B. A. 5 nM of recombinant protein were incubated with 10 nM of Poly(A) at 37°C for 5 minutes in a reaction buffer with different Mg²⁺concentrations.C. Determination of the protein activity in the presence of different Mg²⁺ concentrations.