Figure 5. Exoribonucleolytic activity at 37°C of Synechocystis protein: comparison with E. coli RNase II and RNase R.

Activity assays were performed using the three synthetic substrates: 30-mer poly(A), the 16-mer and the double-stranded substrate 16–30 ds. The concentration of proteins used is indicated in the figure. Samples were taken during the reaction at the time points indicated. Control reactions with no enzyme added (Ctrl) were incubated at the maximum reaction time for each protein. Length of substrates and degradation products are labelled.