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. 2012 Mar 5;7(3):e32690. doi: 10.1371/journal.pone.0032690

Table 1. Kinetic parameters of RNase II-like protein from Synechocystis sp.

ka (1/Ms) kd (1/s) KD (nM)
PolyA 3,0±0,4 E03 1,9±0,1 E-05 3,9±0,3
25 ss 3,7±0,4 E03 1,1±0,1 E-05 3,3±0,5
16–25 ds 2,4±0,2 E05 1,4±0,2 E-04 2,1±0,1

The kinetic constants were determined by Surface Plasmon Resonance using Biacore 2000 with a 25-nt RNA oligomer (5′-Biotin-CCCGACACCAACCACUAAAAAAAAA-3′) and 30 nts PolyA as substrates.