Table 1. Kinetic parameters of RNase II-like protein from Synechocystis sp.
ka (1/Ms) | kd (1/s) | KD (nM) | |
PolyA | 3,0±0,4 E03 | 1,9±0,1 E-05 | 3,9±0,3 |
25 ss | 3,7±0,4 E03 | 1,1±0,1 E-05 | 3,3±0,5 |
16–25 ds | 2,4±0,2 E05 | 1,4±0,2 E-04 | 2,1±0,1 |
The kinetic constants were determined by Surface Plasmon Resonance using Biacore 2000 with a 25-nt RNA oligomer (5′-Biotin-CCCGACACCAACCACUAAAAAAAAA-3′) and 30 nts PolyA as substrates.