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. 2012 Mar 5;7(3):e32749. doi: 10.1371/journal.pone.0032749

Figure 2. Kinetic models of the glyoxalase pathway.

Figure 2

In model 1 (A), glutathione (GSH) and methylglyoxal (MGO) form a hemithioacetal (HTA) which is the substrate of glyoxalase I. In model 2 (B), glutathione and methylglyoxal are sequential substrates of glyoxalase I and the hemithioacetal is formed at the active centre of the enzyme. Glyoxalase II is a one-substrate-one-product irreversible Michaelis Menten enzyme, catalyzing the hydrolysis of S-D-lactoylglutatione (SDLGS) into D-lactate (D-Lac) and glutathione. The rate laws assumed in the models are expressed in equations 15 to 18.