Table 1. Kinetic parameters for S. cerevisiae glyoxalase I and bovine liver glyoxalase II.

Enzyme	Parameter	Value ± Standard error
Glyoxalase I, model 1	k cat GLOI,1	(8±3.0)×104 min−1
	K m,HTA	(0.2±0.14) mM
Glyoxalase I, model 2	k cat GLOI,2	(1.7±0.38)×105 min−1
	K m,GSH	(0.87±0.11) mM
	K m,MGO	(1.2±0.19) mM
Glyoxalase II	k cat GLOII	(3±2.2)×102 min−1
	K m SDLGS	(3±2.1) mM
Non-enzymatic reaction	kf	0.34 mM−1 min−1
	kr	1.01 min−1

Parameters were estimated by collective fit to time-course data, as detailed in Methods, except for the non-enzymatic reaction rate constants, for which previously reported values were used [29]. Dataset S1 includes four time courses used in the estimation of glyoxalase I parameters and four time courses used in the estimation of glyoxalase II parameters.