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. 2012 Mar 5;7(3):e32697. doi: 10.1371/journal.pone.0032697

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Pérez-Redondo et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Cluster of genes for arginine biosynthesis (black arrows) in S. coelicolor M145 (above) and the S. coelicolor ΔargR mutant (below). SCO1581 is indicated with broken lane since it might not be a real gene. (B) Arginine biosynthesis pathway showing its relation to proline and to pyrimidine biosynthesis via carbamoyl-phosphate incorporation. (C) Expression of the arginine biosynthesis genes in A, B, C and D conditions as indicated in Fig. 3. (D) RT-PCR amplification of the argC, argG, argH and argR genes in the same conditions and strains as above. M, Markers.