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. 2012 Mar 5;7(3):e31052. doi: 10.1371/journal.pone.0031052

Figure 2. AR-dependent tumor suppression was associated with increased cellular senescence.

Figure 2

AR increased cellular senescence in vivo. (A) Experimental design. Male athymic mice (nu/nu, 5 mice/group) were injected s.c. with vector control (PC3-V) or PC3-AR cells and received Dox with drinking water, where indicated, to induce AR expression. Flutamide was given to counteract endogenous testosterone. (B) Mean tumor volumes and standard deviations are shown at 45-day time point. Note lower volume of PC3-AR tumors in male mice treated with Dox, and the lack of the decrease when Fl was added to the treatment. (C) Sections of frozen tumors collected in the experiments above were subjected to SA-βGal assay to detect senescence. Note increased βGal positivity (blue) in PC3-AR tumors treated with Dox and of LNCaP tumors treated with DHT.