Figure 9.

RuvA inhibits WRN-stimulated FEN-1 cleavage of Holliday junction by blocking WRN branch-migrating activity. Reactions (20 μl) containing 2.5 fmol of HJ(X12-1) and WRN (12 nM) in the presence or absence of FEN-1 (29 nM) and increasing amounts of RuvA (0-40 nM) were incubated at 37°C under standard conditions. (A) Phosphorimage of a typical native gel is shown. Filled triangle, heat denatured substrate control. (B) Helicase (○) and cleavage (•) activities are expressed as percent of control reactions in which RuvA was omitted.