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. 2012 Mar 5;7(3):e32247. doi: 10.1371/journal.pone.0032247

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Ru et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Sera were collected 10 days after the third immunization, and the titers of the antibody (IgG) against HBs particles in the immunized mice (solid bar) were determined and compared with the mean responses of six PBS-immunized mice (hollow bar) in an ELISA assay. (B) HBs epitope-specific IFN-γ production by CTLs was determined by measuring response to the HLA-A2-restricted epitopes HBsAg348–357 and HBsAg335–343 and to the H-2 K^b-restricted epitope HBsAg371–378 in immunized mice (solid bar) and PBS-immunized mice (hollow bar).