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Specificity of PIM-stimulated endosomal fusion. (A) Fusion was conducted in the presence of 40 μg/ml mycobacterial lipids (top) or 80 μg/ml lipids (bottom). (B) Fusion was conducted for 30 min, in the presence of 80 μg/ml PIM and 2 mg/ml cytosol, by using early endosomes (EE) or late endosomes (LE) loaded with biotinylated-HRP or avidin. Bars, SEM asterisk, p < 0.05 (ANOVA).
