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. 2012 Mar 5;7(3):e32905. doi: 10.1371/journal.pone.0032905

Figure 1. Differential regulation of PSA and IL-8 by NF-κB in LNCaP cells.

Figure 1

To manipulate NF-κB function, RelA and RelB cDNA constructs (A), RelA and RelB siRNAs (B), and RelA and RelB nuclear translocation inhibitors (IκBαM and p100M) (C) were transfected into LNCaP cells prior to IR treatment. The levels of transfected proteins and the related target PSA were quantified by Western blots (top panel). The Western images were normalized by β-actin and then normalized by a vehicle control. Fold changes are indicated. IL-8 concentrations in media were quantified by an Elisa kit (middle panel). Cell survivals were quantified using Trypan blue exclusion assay (bottom panel). * (p<0.05) and ** (p<0.01) indicate statistical significances as compared to the vehicle control.