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. 2012 Mar 5;7(3):e32905. doi: 10.1371/journal.pone.0032905

Figure 2. Reverse correlation of PSA and IL-8 in PCa cells.

Figure 2

To manipulate PSA and IL-8 in PCa cells, LNCaP cells were transfected with an IL-8 cDNA construct and a PSA siRNA (A); PC3 cells were transfected with PSA cDNA construct and IL-8 siRNA (B). To test whether PSA influences NF-κB transcriptional activation, a NF-κB-luciferase reporter construct was co-transfected with a PSA expression construct or PSA siRNA and a β-gal expression construct into LNCaP cells. The luciferase responses were normalized with β-gal activities (C). PSA and IL-8 levels were quantified by Western blots with normalization as described in Figure 1.