Figure 4. The effect of overexpression of RelB on radioresistance of LNCaP tumors.

A stable RelB expressed LNCaP clone was characterized by Western blots (A). The RelB/LNCaP cells were injected into male mice for tumor formation and growth reaching to 500 mm³ (B). The tumors were treated IR (5×3 Gy) and tumor volumes were measured until they reached 2000 mm³ (C). RelB, PSA and IL-8 levels were quantified in the tumor lysates and statistically analyzed (D). Statistical significances of tumor volumes (C) and the levels of the target proteins (D) between RelB expressed and vehicle control in both no- IR and IR treated groups are indicated.