Figure 5. The effect of knock-down RelB on radioresistance of PC3 tumors.

A lentivirus-based RelB siRNA knock-down PC3 clone was characterized by Western blots (A). The RelB shRNA/PC3 cells were injected into male mice for tumor formation (B). The tumors were treated with IR (3×5 Gy) when tumor volumes reached 500 mm³ and tumors were allowed to grow to 2000 mm³ (C). RelB and IL-8 levels were quantified in the tumor lysates and statistically analyzed (D). Statistical analysis for tumor volumes and the levels of target proteins as described in Figure 4.