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. 2012 Mar 5;7(3):e33100. doi: 10.1371/journal.pone.0033100

Figure 3. Reproducibility of mutant allele burden determination by the qPCR assays.

Figure 3

Histogram plots showing reproducibility of percentage mutant allele burden in six separate qPCR runs. The mutant allele burden was determined for 11 different DNA samples from four different patients as indicated. The JAK2 exon 12 mutations include K539L (PV6), V536-I546dup11 (PV4), and N542-E543del (PV1 and PV2) in high, intermediate and low levels of mutant allele burden. The data is presented as percentage mean values ± standard deviation (SD).