Table 1. Primer and probe sequences for JAK2 exon 12.

Primers	Sequence 5′-3′
JAK2exon12-Forw	CAGATAAATCAAACCTTCTAGTCTTC
JAK2exon12-wt-Rev	CAAATCTTCATTTCTGATTTTGTtA
JAK2exon12-N542-E543del-Rev	TGACTTACAAATATCAAATCTCTGA
JAK2exon12-E543-D544del-Rev	CAAATCTTCATTTCTGATTAAGTtA
JAK2exon12-K539L-Rev	CAAATCTTCATTTCTGATTAAGTtA
JAK2exon12-H538-K539delinsL-Rev	AAATATCAAATCTTCATTTCTGATTAAA
JAK2exon12-I540-E543delinsMK-Rev	CAAATATCAAATCTTTCATTTTGTtA
JAK2exon12-H538QK539L-Rev	CAAATCTTCATTTCTGATTAATTtA
JAK2exon12-R541-E543delinsK-Rev	CAAATATCAAATCTTTGATTTTGTtA
JAK2exon12-F537-K539delinsL-Rev	CAAATCTTCATTTCTGATTAACACtA
Primers only for quantitative determination
JAK2exon12-wt (N542-E543del)-Rev	AATGACTTACAAATATCAAATCTTCA
JAK2exon12-V536-I546dup11-Rev	TTTGTGAAACACTATCAAATCTTC
JAK2exon12-F537-I540delinsLV-Rev	AAATATCAAATCTTCATTTCTCACTA
Probe
JAK2exon12 probe	Fam-CCAACCTCACCAACATTACAGAGGCC-Tamra

Primers used for qPCR screening and quantitative determination consisting of a common forward primer and probe in addition to a mutation specific reverse primer. The primers listed for quantitative determination were only used exclusively for determination of mutant allele burden. Lowercase letters in sequences indicate intended mismatches.