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. Author manuscript; available in PMC: 2013 Mar 15.
Published in final edited form as: J Immunol. 2012 Feb 6;188(6):2905–2913. doi: 10.4049/jimmunol.1102595

Figure 4. Tbc1d23 overexpression does not decrease TNFα RNA stability.

Figure 4

Either RAW264.7 cells (solid black line) or cells overexpressing Tbc1d23 (dashed grey line) were stimulated with 50 ng/ml LPS for two hours, actinomycin D was subsequently added to inhibit further transcription, and cells were lysed in RLT buffer (Qiagen) at the indicated times after stimulation. TNFα mRNA levels were monitored by qPCR and normalized relative to the RAW264.7 cells at time 0.