Fig 4.

Locomotory activity, quantified as the number of times fish crossed the lines of a 3 × 3 virtual grid (the ambulation score) is reduced by the presentation of the stimulus. Mean ± S.E.M. are shown. The data are expressed for three intervals separately, the pre-stimulus period (the first interval during which no stimulus was shown), the stimulus period (during which the moving black bird silhouette was presented multiple times), and the post-stimulus period (during which no stimulus was presented. The shading of the bars corresponds to the alcohol dose used with darker shades indicating higher concentrations (see legends). Note the apparent, but non-significant, alcohol concentration dependent linear trend, especially during the pre-stimulus interval, towards reduced activity. For details of the results of statistical analyses see Results.