Table 2.
Half-time (min) of the internalization step of endocytosis of GFP-tagged uracil permease derivatives
Strains | Fur4p | Fur4KRp | Ub-Fur4p | Ub-Fur4KRp |
---|---|---|---|---|
WT | 40 | >180 | 20 | 75 |
npil | >180 | >180 | 75 | 70 |
Uracil uptake was measured various times after the addition of cycloheximide and the time corresponding to a 50% loss of activity is reported.