Figure 1. Acetylation of the MuLV IN protein.
Acetylation of MuLV IN with p300 catalytic domain was performed as described in Materials and Methods. Protein samples were subjected to SDS-PAGE and stained with Coomassie blue (left) or subjected to autoradiography (right). Lanes 1, MuLV IN 1-407; Lanes 2, MuLV IN 1-397; Lanes 3, BSA; Lanes 4, histone control. Position of migration of prestained molecular weight broad range markers (BioRad) are indicated.