Figure 4. qPCR of replication products and Alu-PCR.
(A) qPCR of replication products. Graph plots the fold-increase in the amount of PCR products relative to WT virus at 24 h post infection for minus strand strong stop (MSSS), plus strand extension (PSE) and 2-LTR circle products. (B) Alu-PCR. Graph plots the fold decrease in integrated provirus for IN mutants relative to WT IN at 18 days post infection. Results are mean with standard error, N=3. Statistical significance was determined by one-way ANOVA (***, P < 0.001; **, P < 0.01; *, P < 0.05; n.s., not significant).