Figure 7. Temporal requirement for TSA effect on transgene expression.
(A) Comparison of cells infected with WT and IN K376R mutant virus and treated with the HDAC inhibitor TSA at various time points. Untreated, no TSA added; Pre-treatment, TSA added 12 h prior to infection; Time of, TSA added with viral infection; Post-treatment, TSA added 48 h post infection. GFP was measured by flow cytometry at 3 dpi. Cell number is represented along the y-axis, and GFP intensity is measured on the x-axis.