Figure 2.
Figure 2A: Motor activity of ecdysoneless1 females. Ecdysone-depleted female flies are less active. The same ecdysoneless1 females treated at 29°C for 12 hours as in Figure 1 were analyzed for motor activity by measuring the frequency at which they cross an arbitrary line at the center of their otherwise empty chamber. ecdysoneless1 females were significantly less active than wild-type controls, and when their diet contained 10−4 M 20E, their activity was further reduced. Ecdysteroid supplementation of the diet of wild-type control females did not significantly affect motor activity. P values are from Student t-test. Whiskers indicate standard error of the mean.
Figure 2B: Attractiveness of decapitated females. The non-behavioral attractiveness of mutant and wild-type females was assessed by measuring the level of courtship they elicit following decapitation. The time wild-type male suitors spent performing unilateral wing extension, licking, tapping, and attempting copulation was measured in a 10-minute period and an attractiveness index was expressed as duration of courtship divided by total time. No significant differences were observed. Whiskers indicate standard error of the mean.