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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Proteins. 2012 Jan 7;80(4):1095–1109. doi: 10.1002/prot.24010

Figure 4. Fluorescence anisotropy assay used to measure peptide binding to RARα ligand binding domain.

Figure 4

The binding measurements were performed at 20 °C and pH 8.1, as described in “Materials and Methods”. All measurements were repeated with at least two independent protein preparations. The results from a representative experiment are shown. The main plot depicts an experiment with wild type RARα LBD and a peptide based on the sequence of the N-CoR nuclear co-repressor. The inset depicts a fluorescence anisotropy experiment employing a scrambled sequence of the N-CoR peptide as ligand.