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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Proteins. 2012 Jan 7;80(4):1095–1109. doi: 10.1002/prot.24010

Figure 7. Thermodynamic effect of co-factor binding on mutant ATRA affinity.

Figure 7

ATRA concentration is plotted versus the log of the KD for each RARα LBD to N-CoR or ACTR. A, ATRA binding affinity is largely unaffected by N-CoR binding. With the exception of M297L, affinity to ATRA does not differ significantly amongst the N-CoR-bound receptors, visualized by the similarly shaped ATRA-response curves. B, The presence of ACTR, however, has a large effect on ATRA binding affinity. The mutations, particularly L398P and I410T, weaken the ATRA affinity of ACTR-bound receptors, visualized by the diverse shapes of the ATRA-response curves.